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Part:BBa_K2150020

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+GFP+RBS+T7RNAP+DT

By adding a T7 RNA polymerase(T7RNAP) coding region before terminator, T7RNAP can be produced and further activate pT7, resulting in an increased expression of GFP. This part can be used to achieve positive feedback effect, leading to the over expression of a target protein(GFP in this case).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


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